Harvesting of DCs - (Nov/07/2008 )

Hi everybody,

we tried to isolate dendritic cells from rat bone marrow for first time, but we had problems with harvesting them. Acording to the protocol we tried to dislodge them with pipette, but they were strong adherent on the surface of the flask. We also used trypsin but it didn't help, they were still in the flask. We haven't any experiences with culturing of DCs, could someone help us?

Thank you very much

may use a 1 or 2ml pipette. They are better than 10 ml for dislodging the DC from the flask.