temperature can effect the protein active? - (Oct/29/2008 )
I wan to express one protein in Ecoli, I select 27 degree 12 hours, 37 degree 4 hours and 16 degree 14 hours. who can tell me if there is different between them, and I found that different temperature, the amount of expressed protein was different. which situation is better for protein active, and I want to do in vitro test?
thanks at first!
-willmen-
QUOTE (willmen @ Oct 29 2008, 11:00 PM)
I wan to express one protein in Ecoli, I select 27 degree 12 hours, 37 degree 4 hours and 16 degree 14 hours. who can tell me if there is different between them, and I found that different temperature, the amount of expressed protein was different. which situation is better for protein active, and I want to do in vitro test?
thanks at first!
thanks at first!
if iti s heterologue expression, it depends on species; a marine bacterium protein has other needs for temperature than mammalian cell protein
-The Bearer-