Changing the pH of liquid e.Coli cultures. - (Oct/23/2008 )

For a science fair project, my partner and I need to know how to change the pH level of liquid e.Coli cultures. We don't want to dilute the e.coli with different pH solutions, so adding a set amount of pH 3 water is out. Any suggestions?
-- Thank you

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What do you want to do with the different pH broths?

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In the end we are going to sequence the different cultures in order to determine if environmental stress increases the rate of mutation. Throughout the experiment we are also going to measure the time it takes for the e.coli to reach a certain density, to see how fast/well it is adapting.
However, our knowledge on liquid cultures is severely limited, as we have only worked with agar plates.

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sequencing the culture ?! holy smoke .. like the whole genome or what..
adding NaOH or HCl would be what i would do to adjust the pH of broth.

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You might be able to grow them under unaerobic conditions. That should change the pH. Or just add high concentrations of an acid or a base. this way you dont really dilute the culture. NaOH pellets would be another possibility.

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Look up buffers.

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