finding transposon signatures in sequence - transposons (Oct/21/2008 )
Dear all,
I have found a mutant allele of my gene of interest and am trying to characterize the mutation and its effect on transcription and translation of my gene.
The interesting thing is that the 3' end of my gene is missing. I suspect that what I am seeing is a retroelement that inserted into my gene and then left taking with it the 3' end of my exon.
Does anyone know how one can recognize transposons once they have left a gene. What is the signature that they leave? Are there programs out there that can help me spot those signatures?
Thanks!
-genejockey-
Why don't you suspect it is caused by nonsense mutation? I believe it is more common and is easy to be detected
QUOTE (genejockey @ Oct 21 2008, 08:02 AM)
Dear all,
I have found a mutant allele of my gene of interest and am trying to characterize the mutation and its effect on transcription and translation of my gene.
The interesting thing is that the 3' end of my gene is missing. I suspect that what I am seeing is a retroelement that inserted into my gene and then left taking with it the 3' end of my exon.
Does anyone know how one can recognize transposons once they have left a gene. What is the signature that they leave? Are there programs out there that can help me spot those signatures?
Thanks!
I have found a mutant allele of my gene of interest and am trying to characterize the mutation and its effect on transcription and translation of my gene.
The interesting thing is that the 3' end of my gene is missing. I suspect that what I am seeing is a retroelement that inserted into my gene and then left taking with it the 3' end of my exon.
Does anyone know how one can recognize transposons once they have left a gene. What is the signature that they leave? Are there programs out there that can help me spot those signatures?
Thanks!
-buyao0-