Calculation issue for inconsistent results in triplicates in Taqman rPCR. - (Oct/21/2008 )
QUOTE (Trof @ Oct 31 2008, 06:36 PM)
QUOTE (rPCR7 @ Oct 31 2008, 05:56 AM)
Guess this method is good for research only. If it is applied to a clinical test, the test fees might be another issue since each sample will consume a lot of reagents.
Actually I think the main use of this is to estimate sensitivity of an assay, so it should be performed once before putting a new diagnostic assay to use. You need to know how sensitive your reaction is, especially in diagnostics. Of course in routine testing you wouldn't set up 20 reactions, but you can tell the sensitivity treshold.
Trof,
Okay, got your point. Thank you again for the further discussion.
rPCR7.
-rPCR7-