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Leu-rich repeat - help (Oct/01/2008 )

hi

anyone can give me the example of Leu-rich repeat?or the sequences which they maybe?

Stone

-stone757-

hi stone,
this like has answers to all your questions smile.gif

http://en.wikipedia.org/wiki/Leucine-rich_repeat

cheers,

tj

-toejam-

QUOTE (toejam @ Oct 1 2008, 10:57 PM)
hi stone,
this like has answers to all your questions smile.gif

http://en.wikipedia.org/wiki/Leucine-rich_repeat

cheers,

tj


Hi toejam

It is a long time that we have not seen each other.

thanks very much for the website.

However, how can I analysis my sequences and to judge whether they have LRR in it?

Regards
Stone

-stone757-

first of all you could do a blast to a protein database (aka nr in ncbi), that should tell you if there are any leucine-rich domains.
there's another webpage that works to detect protein motifs. i can't remember right now, but i'm sure i've seen it in a post in this subforum.
else, you could do a six frame translation of your nucleotide sequences and look for leucines...i wouldn't do that, sounds too extensive, unless your sequences are short and you don't have too many.

-toejam-

QUOTE (toejam @ Oct 1 2008, 11:11 PM)
first of all you could do a blast to a protein database (aka nr in ncbi), that should tell you if there are any leucine-rich domains.
there's another webpage that works to detect protein motifs. i can't remember right now, but i'm sure i've seen it in a post in this subforum.
else, you could do a six frame translation of your nucleotide sequences and look for leucines...i wouldn't do that, sounds too extensive, unless your sequences are short and you don't have too many.



ok i see. really thanks very much.

Regards

Stone

-stone757-