Immunization with inclusion bodies - (Sep/25/2008 )

Hello everybody.

I got my protein in inclusion bodies, and I wonder if I could just purify and use them for subcutaneous immunization of rabbits. Could I alternatively solubilize bodies in 6 M urea and use that solution directly to immunize?

I also wonder if the same could be used for intraperitoneal injection in mice to get monoclonal antibodies.

Thanks!

hi,

u will have antibodies against ur inclusion bodies, once u have these antibodies where do u want to use? to detect inclusion bodies? if u r lucky if dominant epitope is not hiding, there may be a possibility of getting specific antibodies against ur protein, but it is too risky...

heavy concentration of urea to give SC or IP routes, it depends on volume of urea solution (antigen) u immunize. dialyze your samples and use for immunizations.