Negative Control Rabbit IgG for ChIP Assay - (Sep/23/2008 )
I am going to use Rabbit polyclonal Antibodies to do ChIP experiment on mouse tissues
And I'd need a negative control for the assay, which should be a Rabbit IgG.
I searched briefly on Upstate and couldn't find one on their homepage
Does anyone have any good experience using a particular rabbit IgG as negative control?
If possible, I would love to know the catalog number so that I can order it directly.
Thanks a lot~!!
-jiro_killua-
QUOTE (jiro_killua @ Sep 23 2008, 05:20 PM)
I am going to use Rabbit polyclonal Antibodies to do ChIP experiment on mouse tissues
And I'd need a negative control for the assay, which should be a Rabbit IgG.
I searched briefly on Upstate and couldn't find one on their homepage
Does anyone have any good experience using a particular rabbit IgG as negative control?
If possible, I would love to know the catalog number so that I can order it directly.
Thanks a lot~!!
And I'd need a negative control for the assay, which should be a Rabbit IgG.
I searched briefly on Upstate and couldn't find one on their homepage
Does anyone have any good experience using a particular rabbit IgG as negative control?
If possible, I would love to know the catalog number so that I can order it directly.
Thanks a lot~!!
I think I get mine from Sigma, but I can check when I go to work
Clare
-Clare-
hi,
i used normal rabbit IgG from a chIP kit (upstate). it works excellent and i have very bad experience with normal rabbit IgG for my target protein.
the catlog number they have given is PP64B/
kit catlog number is 17-408.
gud luk
QUOTE (jiro_killua @ Sep 23 2008, 06:20 PM)
I am going to use Rabbit polyclonal Antibodies to do ChIP experiment on mouse tissues
And I'd need a negative control for the assay, which should be a Rabbit IgG.
I searched briefly on Upstate and couldn't find one on their homepage
Does anyone have any good experience using a particular rabbit IgG as negative control?
If possible, I would love to know the catalog number so that I can order it directly.
Thanks a lot~!!
And I'd need a negative control for the assay, which should be a Rabbit IgG.
I searched briefly on Upstate and couldn't find one on their homepage
Does anyone have any good experience using a particular rabbit IgG as negative control?
If possible, I would love to know the catalog number so that I can order it directly.
Thanks a lot~!!
-Dr.House-
QUOTE (Dr.House @ Sep 26 2008, 05:20 AM)
hi,
i used normal rabbit IgG from a chIP kit (upstate). it works excellent and i have very bad experience with normal rabbit IgG for my target protein.
the catlog number they have given is PP64B/
kit catlog number is 17-408.
gud luk
i used normal rabbit IgG from a chIP kit (upstate). it works excellent and i have very bad experience with normal rabbit IgG for my target protein.
the catlog number they have given is PP64B/
kit catlog number is 17-408.
gud luk
QUOTE (jiro_killua @ Sep 23 2008, 06:20 PM)
I am going to use Rabbit polyclonal Antibodies to do ChIP experiment on mouse tissues
And I'd need a negative control for the assay, which should be a Rabbit IgG.
I searched briefly on Upstate and couldn't find one on their homepage
Does anyone have any good experience using a particular rabbit IgG as negative control?
If possible, I would love to know the catalog number so that I can order it directly.
Thanks a lot~!!
And I'd need a negative control for the assay, which should be a Rabbit IgG.
I searched briefly on Upstate and couldn't find one on their homepage
Does anyone have any good experience using a particular rabbit IgG as negative control?
If possible, I would love to know the catalog number so that I can order it directly.
Thanks a lot~!!
Thanks,
I asked Upstate and they also recommended pp64, seems like a good choice
BTW, what do you mean by "have very bad experience with normal rabbit IgG for my target protein"?
-jiro_killua-