Pseudomonas aeruginosa - difficult to culture? - and other questions (Sep/22/2008 )

anyone experienced with P. aeruginosa? How to culture? Which strain is commonly used? What is the common cell model to study infection?
any hint or recommendation is very welcome

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Pseudomonas will grow well on tryptic soy agar with 5% sheep blood (regular blood agar plate) It also grows well on Mueller-Hinton agar and will be bright green/neon yellow. It smells like grapes too. I'm not sure what common strain is used, but I assume it is whatever ATCC strain used for QC in a clinical micro lab. You could probably use isolated macrophages to study infection.

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most of the people use P.aerginosa ATCC 27583 strain for the analysis. you can grow the bacteria on TH plates, but for better results , it is better to use Blood agar plates

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The usual strain is Pseudomonas aeruginosa PAO1 (ATCC 15692). It grows well on L-agar, too. If you want to confirm Pseudomonas aeruginosa, use cetrimide agar, but for routine culturing, L-agar and L-broth are fine. If you're using a plasmid with Amp resistance, select on carbenicillin at 500 ug/ml.

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