Protocol Online logo
Top : Forum Archives: : Cell Biology

Do you dissolve DAPI in water or methanol? - or PBS? (Sep/07/2008 )

Do I need to dissolve my DAPI in water? .........I've got 5mg powder from Sigma, but don't know to dissolve in what solution!!!

-Curtis-

We used to make up DAPI in PBS.

-scolix-

QUOTE (scolix @ Sep 7 2008, 07:07 AM)
We used to make up DAPI in PBS.


thanks, but as far as I'm concerned it won't dissolve in PBS.

-Curtis-

We use ours in PBS too!


QUOTE (Curtis @ Sep 7 2008, 04:58 PM)
QUOTE (scolix @ Sep 7 2008, 07:07 AM)
We used to make up DAPI in PBS.


thanks, but as far as I'm concerned it won't dissolve in PBS.

-scistudent-

What does Sigma suggest? If they suggest water, try water.

-scolix-

We dissolve in water!

-skyyingkai-

QUOTE (scolix @ Sep 7 2008, 08:07 AM)
We used to make up DAPI in PBS.

So do I. Same thing from Sigma. It works perfectly all right.

-Twisters-