suspension cells - getting them to settle (Sep/03/2008 )

Hi,

Does anyone use suspension cells? i'm not used to using them but will be soon. at some point in my work i'm going to want to stick them on a culture slide. is there a method for getting suspension cells to stick to a slide?

thanks for any advice

hodge

[quote name='hodgehegs' date='Sep 3 2008, 08:31 AM' post='149361']
Hi,

Does anyone use suspension cells? i'm not used to using them but will be soon. at some point in my work i'm going to want to stick them on a culture slide. is there a method for getting suspension cells to stick to a slide?
thanks for any advice


hodge


I assume you want to do immuno on the cells at some point. This could be a problem with suspension cells. Most one's I have used are purely suspension cultures and will not normally stick to plastic. However you can try and use attachment factors such as:-
Polylysine
Fibronectin
Collagen
Primaria
Laminin
Gelatin


You could also try another immuno approach and label cells and use FACS analysis instead

Kindest regards

Rhombus

it is indeed so we can do some immuno at a later point. we were thinking of putting the cells in serum free media. is it possible that would work, as have heard it might. not too keen to go down the facs route.

HI,
we culture cells on serum-free media with-out problems...
and we put them on slices and seems to growth fine, but what kind of cells are you using??? We culture them on coverslips up to confluence and then, after a passage, drop an aliquot on the slice (and put the rest on new coverslip)
May be I didn't understud you well

Seba