How to preserve blood serum? - (Aug/29/2008 )
Hello everybody!
Could anyone please tell me how to keep blood serum some days before it being preserved in liquid nitrogen at -196ºC?
I think it can be kept in the fridge during 3 days and after that at -20ºC. Can I thaw it and freeze again until -196ºC?
Thanks.
Luísa
-alpere-
QUOTE (alpere @ Aug 29 2008, 01:55 PM)
Hello everybody!
Could anyone please tell me how to keep blood serum some days before it being preserved in liquid nitrogen at -196ºC?
I think it can be kept in the fridge during 3 days and after that at -20ºC. Can I thaw it and freeze again until -196ºC?
Thanks.
Luísa
Could anyone please tell me how to keep blood serum some days before it being preserved in liquid nitrogen at -196ºC?
I think it can be kept in the fridge during 3 days and after that at -20ºC. Can I thaw it and freeze again until -196ºC?
Thanks.
Luísa
What we do is freeze it at -80oC exactly after the isolation from whole blood. We don't freeze it at liquid nitrogen.
Good luck..
-doctorT-
I agree with doctorT. Never heard about liquid nitrogen for serum 
-Pumuki-
QUOTE (alpere @ Aug 29 2008, 07:55 PM)
Can I thaw it and freeze again?
Keep in -80degC and thaw-freeze not more than once.
-Bungalow Boy-