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protein extraction fromn soil bacterium - (Aug/01/2008 )

can any one please suggest the protocol for protein extraction from soil bacterium.
i want to isolate extracellular as well as total (extracellular and intracellular) proteins

thanks

-coffee-

QUOTE (coffee @ Aug 1 2008, 08:23 AM)
can any one please suggest the protocol for protein extraction from soil bacterium.
i want to isolate extracellular as well as total (extracellular and intracellular) proteins

thanks


have you checked these links:


http://www.biochemj.org/bj/030/0599/0300599.pdf
http://www.protocol-online.org/cgi-bin/pro...che.cgi?ID=3258
http://www.protocol-online.org/cgi-bin/pro...che.cgi?ID=3259
http://www.protocol-online.org/cgi-bin/pro...in%20extraction

-pito-