Sectioning - (Jul/22/2008 )

Hi,

Any good way to cut to form a flat piece of specimen beside microtoming? Any special techniques involved?

Thanks

-watertreader-

freeze it

you can embed in goo and use a cryostat or simply freeze in liq nitrogen and use a razor blade (good for bits of spine as i remember)

-Dominic-

QUOTE (watertreader @ Jul 22 2008, 11:45 PM)

Hi,

Any good way to cut to form a flat piece of specimen beside microtoming? Any special techniques involved?

Thanks

how thick do you want the sections?

-scolix-

QUOTE (scolix @ Jul 23 2008, 07:35 AM)

how thick do you want the sections?

Free size... Thickness could be quite thick...above 500um or close to 1mm

-watertreader-

QUOTE (watertreader @ Jul 23 2008, 10:11 PM)

QUOTE (scolix @ Jul 23 2008, 07:35 AM)

how thick do you want the sections?

Free size... Thickness could be quite thick...above 500um or close to 1mm

How about using vibrotome?

-scolix-

QUOTE (scolix @ Jul 24 2008, 07:32 AM)

QUOTE (watertreader @ Jul 23 2008, 10:11 PM)

QUOTE (scolix @ Jul 23 2008, 07:35 AM)

how thick do you want the sections?

Free size... Thickness could be quite thick...above 500um or close to 1mm

How about using vibrotome?

Would there be a good manual technique? Do not have to be accurate but would just require a certain degree of flatness

-watertreader-