His Antibody - Is any worked with 4xHIs tag protein (Jul/18/2008 )
I cloned my protein with His Tag (four His). Do anybody worked with or know whether antibody for 6xHis will also recoginise 4His or there is any commercially antibody which recogonise 4xHis.
-bhappy-
HI
HIS antibody will recognize both 4x and 6x , so dont worry run a westrn and see
correct me if u get wrong results
regards
s
QUOTE (bhappy @ Jul 19 2008, 04:04 AM)
I cloned my protein with His Tag (four His). Do anybody worked with or know whether antibody for 6xHis will also recoginise 4His or there is any commercially antibody which recogonise 4xHis.
-shan-