collagen inhibitor (or a way to remove it) - (Jul/17/2008 )
Hello everybody, I'm looking for a way to remove collagen from a platelet suspension. I explain: I have got a platelet suspension with collagen and a drug, now I must remove collagen but I can't centrifuge because platelets will activate with a centrifugation and I absolutely don't want they activates! I'm looking for a collagen inhibitor or another way to remove it. I have got some collagenase I don't know if it will work for this purpose and I don't know how use it!! Please help!!!!
How about collagenase digestion? The drawback is that collagenase needs Ca, which may activate your platelets. Look up the necessary concentrations.
Hello Kupac, I usually use collagenase to detach endothelial cells from the umbelical vein... may I use the same collagenase? Where can I find the necessary concentration? I tried to do a pubmed but I didn't find anything!
Necessary [Ca++] for the collagenase should be on the product datasheet. Some books/papers would tell you what [Ca++] you need to activate platelets. If the first one is much lower, you may be lucky. If it is much higher, then it does not make sense to try. You can try to play around with it yourself, unless someone has a better idea.
So I will try with collagenase even if I have not idea how to use it....