Fixation - How do we determine if fixation is done in right manner (Jul/13/2008 )

Hi,

Just wondering on how do we determine that fixation is done optimally. I have tried to do fixation by 4% paraformaldehye on chicken cartilage sample in either 2 days or even 7 days. There seem to be not much of a difference in the end product.

Is there any way to quantify or qualify the end result?

Thanks

Hi,

Just wondering on how do we determine that fixation is done optimally. I have tried to do fixation by 4% paraformaldehye on chicken cartilage sample in either 2 days or even 7 days. There seem to be not much of a difference in the end product.

Is there any way to quantify or qualify the end result?

Thanks

Had the same question about my cell suspension I wanted to fix for experiment. Could not find So took other clues in flow-cytometry - increased background fluoresence compared to unfixed cells. Though, I have no basis to assume that