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SDS-PAGE - (Jul/11/2008 )

one of my sample only has 50ng protein and I want to dissolve it in 10 uL buffer and then run SDS-PAGE. do you think this concentration will work in SDS-PAGE? Will it be too low?

thank you.

-yangstar-

QUOTE (yangstar @ Jul 11 2008, 10:20 AM)
one of my sample only has 50ng protein and I want to dissolve it in 10 uL buffer and then run SDS-PAGE. do you think this concentration will work in SDS-PAGE? Will it be too low?

thank you.


if it is only one protein you will succeed with siver staining; if it is a crude mix of proteins, you will need more protein

-The Bearer-