Method for soil DNA extraction - (Jun/30/2008 )
Can any one suggest me a "good" method of Soil DNA extraction,
also please suggest from where i can get more information on this topic.
thanks
-coffee-
QUOTE (coffee @ Jun 30 2008, 02:08 PM)
Can any one suggest me a "good" method of Soil DNA extraction,
also please suggest from where i can get more information on this topic.
thanks
also please suggest from where i can get more information on this topic.
thanks
There are "good" methods for every problem, but they may vary considerably from problem to problem...sometimes its kits, sometimes home-made protocols....maybe you can tell us more about your soil-DNA problem?
Some literature that might be helpful:
Wilson 1997: http://www.pubmedcentral.nih.gov/articlere...mp;artid=168683 (should be available as free text via PubMed central)
Robe et al. Extraction of DNA from soil, European Journal of Soil Biology 39(4) 2003, Pages 183-190.
(http://www.sciencedirect.com/science/article/B6VR7-49971HB-2/1/83898acf2bcfcefb294bdbd4458544d1)
Abstract:
and more recent: Comparative analysis of environmental DNA extraction and purification methods from different humic acid-rich soils. Authors: Lakay, F.M.; Botha, A.1; Prior, B.A.1, Source: Journal of Applied Microbiology, Volume 102, Number 1, January 2007 , pp. 265-273(9) Publisher: Blackwell Publishing
-gebirgsziege-
QUOTE (coffee @ Jun 30 2008, 05:08 AM)
Can any one suggest me a "good" method of Soil DNA extraction,
also please suggest from where i can get more information on this topic.
thanks
also please suggest from where i can get more information on this topic.
thanks
Many links here:
http://search.vadlo.com/b/q?keys=soil+DNA&...mp;sn=158621799
..
-cellcounter-
Hi
I use a cambio soil extraction kit. They let you have free samples to try.
-taggart-