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Glowing bacteria - (Jun/25/2008 )

Dear all,

Not sure if you guys came across any tv commercial where bacteria can be visualized on any surfaces (table, hands etc.) using UV light. What it is on the surface of the bacteria cell wall that causes this?

According to what I remember from chemistry class on UV/Vis absorption principle, only compounds with double bonds and an unpaired electron will absorb (either UV or visible light) and release the energy so as to appear glowing.

Or is it due to some some compound which gives the bacteria the ability to glow as stated here?

Thanks.

-Dreamchaser-

QUOTE (Dreamchaser @ Jun 25 2008, 04:19 PM)
Dear all,

Not sure if you guys came across any tv commercial where bacteria can be visualized on any surfaces (table, hands etc.) using UV light. What it is on the surface of the bacteria cell wall that causes this?

According to what I remember from chemistry class on UV/Vis absorption principle, only compounds with double bonds and an unpaired electron will absorb (either UV or visible light) and release the energy so as to appear glowing.

Or is it due to some some compound which gives the bacteria the ability to glow as stated here?

Thanks.


I havent got a lot of time now to explain it myself or give more commentary, but I hope this helps:
http://biotech.biology.arizona.edu/labs/Ba...adition_st.html
http://www.pubmedcentral.nih.gov/articlere...gi?artid=372803

-pito-

QUOTE (pito @ Jun 25 2008, 11:56 PM)
QUOTE (Dreamchaser @ Jun 25 2008, 04:19 PM)
Dear all,

Not sure if you guys came across any tv commercial where bacteria can be visualized on any surfaces (table, hands etc.) using UV light. What it is on the surface of the bacteria cell wall that causes this?

According to what I remember from chemistry class on UV/Vis absorption principle, only compounds with double bonds and an unpaired electron will absorb (either UV or visible light) and release the energy so as to appear glowing.

Or is it due to some some compound which gives the bacteria the ability to glow as stated here?

Thanks.


I havent got a lot of time now to explain it myself or give more commentary, but I hope this helps:
http://biotech.biology.arizona.edu/labs/Ba...adition_st.html
http://www.pubmedcentral.nih.gov/articlere...gi?artid=372803


Thanks pito for the links. Yes, I'm aware of bioluminescing by genetic means but then it seems that people detect bacteria like they detect blood and semen using UV.

-Dreamchaser-

QUOTE (Dreamchaser @ Jun 25 2008, 05:24 PM)
QUOTE (pito @ Jun 25 2008, 11:56 PM)
QUOTE (Dreamchaser @ Jun 25 2008, 04:19 PM)
Dear all,

Not sure if you guys came across any tv commercial where bacteria can be visualized on any surfaces (table, hands etc.) using UV light. What it is on the surface of the bacteria cell wall that causes this?

According to what I remember from chemistry class on UV/Vis absorption principle, only compounds with double bonds and an unpaired electron will absorb (either UV or visible light) and release the energy so as to appear glowing.

Or is it due to some some compound which gives the bacteria the ability to glow as stated here?

Thanks.


I havent got a lot of time now to explain it myself or give more commentary, but I hope this helps:
http://biotech.biology.arizona.edu/labs/Ba...adition_st.html
http://www.pubmedcentral.nih.gov/articlere...gi?artid=372803


Thanks pito for the links. Yes, I'm aware of bioluminescing by genetic means but then it seems that people detect bacteria like they detect blood and semen using UV.


I am not sure how widespread that method is, but instead of using bioluminescense, you can be used fluorescense to detect cloned, mutant cells. You can induce a plethora fluorescent proteins, like for example the green fluorescent protein (GFP)

-Synthesis-