Comparative of Genomic and cDNA sequences - Intron sequences!!! (Jun/23/2008 )

To all forum members!

hai, i've been working in sequenced the genomic DNA and cDNA sequences of phytochelatin synthase gene from a seaweed (red algae). when both two sequences were aligned using ClustalW, suprisingly the is no differences in term of nucleotide bases. so, i desperately asking any explanation from anyone whoever have any idea or previously read any journal regarding this matter. any helps are really appreciated...tq.

bullz,
Institute of biology system,
National University of Malaysia,
Malaysia.

i agree with cc, another possible explanation is that your cDNA is contaminated with DNA and that's why you're sequencing the same product

i think that all the condition has been considered. of couse i might be the ist one who isolate the gene from algae (Eucheuma denticulatum) but, all this three years of my research i cannot find any journal or sources that can support my findings. it might less intron gene as you mention...well, i don't know. any journal or evidence to support it is really appreciated.

regarding the genomic contamination, i believe that the technique that was used is able to isolate highly pure of RNA. in fact i have purify again my total RNA using RNA purification column. the cloning was carried 3 times with 3 different time of RNA extraction. its all the same.

hehehe by the way, thank you for the comment!!


bullz,
Institute of biology system (INBIOSIS),
National University of Malaysia,
Malaysia.