Apoptosis without fragmentation - (Jun/19/2008 )

Hi,

I'm looking for cell lines that do not show evidence of oligonucleosomal DNA laddering upon induction of apoptosis.
Preferrably a non-adherent cell line, but any kind would do!

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This is interesting. You want to induce apoptosis, a hallmark of which is DNA laddering, and do not want to see the hallmark!

I am not aware of any such thing, nor do I think such a mammalian cell line would exist, but I can not say that for sure just based on prior logic.

Edit: Madrius just taught me the right spelling for Whole-Mark! Will send you a hallmark card soon

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Well, strange as it may seem, it is a fact that DNA fragmentation is not allways seen in primary cells as well as certain cancer cell lines,
and I was wondering if it was the case for any of the more common cell lines.

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in what cancer cell lines you can't see?

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There are a lot of different mechanisms by witch a cell can die. We often think that necrosis or apoptosis are the two only way, but there are in fact other programmed cell death. They are classified by their different hallmarks. Maybe you should look at those other death methods, rather than cell lines without DNA framentation.

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