difficult to find the transformates containing insert DNA - ligation, transformation (Sep/14/2004 )

Yes. One thousand people said it should work but I just couldn't succeed yet. Right now I try electroporating E. coli with the ligation products to increase the transformation efficiency see if it works. It doesnt' I am going to try different T4 ligase and different ligation ratio. I beleive it should work eventually : . thanks