cytotoxicity of FDA - (Jun/17/2008 )
Hi all,
I used fluorescein diacetate (FDA) to stain the cytoplasm of my cells a few days back, before realising i needed the cells longer. Does FDA cause cytotoxicity to cells, and does it stay in the cytoplasm or leaks out of the cell/gets metabolised? A internet search on cytotoxicity of FDA only gave results in which FDA is used to measure cytotoxicity...
Thank in advance!
-Freebird-
QUOTE (Freebird @ Jun 17 2008, 02:50 PM)
Hi all,
I used fluorescein diacetate (FDA) to stain the cytoplasm of my cells a few days back, before realising i needed the cells longer. Does FDA cause cytotoxicity to cells, and does it stay in the cytoplasm or leaks out of the cell/gets metabolised? A internet search on cytotoxicity of FDA only gave results in which FDA is used to measure cytotoxicity...
Thank in advance!
I used fluorescein diacetate (FDA) to stain the cytoplasm of my cells a few days back, before realising i needed the cells longer. Does FDA cause cytotoxicity to cells, and does it stay in the cytoplasm or leaks out of the cell/gets metabolised? A internet search on cytotoxicity of FDA only gave results in which FDA is used to measure cytotoxicity...
Thank in advance!
For what it's worth: my cells are still alive, are not fluorescent anymore.
-Freebird-