Calculating Relative Expression - how should I analyze my data????? (Jun/12/2008 )

Hello,

I have done qRT-PCR where I have looked at the expression of two separate genes in leukemia samples whose expressions are not the same in the control. I used huPO as my internal control. How do I adjust for this in my analysis? I thought I could just calculate the relative expression and then do a correlation and linear regression and be done. Am I correct or should I do something else?

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First thing you have to do is calculation of expression index (which is equal to delta Ct, the difference of Ct between the target gene and internal control HuPO)for gene A or B in your samples. Then you can calculate correlation using expression index for gene A and B using spearman correlation analysis.

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Thank you so much for your great advice. I was wondering though, is it possible to also do a pearson's and see which is best?

Thanks,

Amy

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If your data belong to normal distribution, it is okay. But based on my experience, spearman is bettter.

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