Ht-29 Subculture - Cell culture (Sep/13/2004 )

when i sub my HT-29s from a 75cm confluent flask using 3ml 1X trypsin:edta and reseed in a new flask the growth is very sluggish and clumpy, there is always a large number of floaters after a few days, i usually feed the cells every 4-5 days? is this enough.

anyone got any ideas

You are most likely over trypsinizing your cells and they are dying. Try using 0.5X trypsin-EDTA and they won't die or be very sluggish. Feed when the media starts to change. Once a week may not be good.