failed Plaque Assay! what do I do wrong? - my plaque assays fail one after another!!! (Jun/01/2008 )
QUOTE (Curtis @ Jun 25 2008, 06:10 AM)
QUOTE (avalon @ Jun 24 2008, 03:54 PM)
It would be better if you dilute the virus in serum free DMEM instead of PBS too
I thought DMEM doesn't let virus attach to host cell surface/receptors, and that is why we wash the cells before adding virus+PBS.
DMEM is just a salt solution like PBS ....its the FBS that can inhibit attachment. You wash the cells to remove the serum.
-avalon-
QUOTE (avalon @ Jun 26 2008, 08:20 AM)
DMEM is just a salt solution like PBS ....its the FBS that can inhibit attachment. You wash the cells to remove the serum.
Thanks, I did as you said today. I have to wait for some days now.
-Curtis-