How to kill yeast cells :) - chloramine or autoclaving (May/27/2008 )
Perhaps you shouldn't be doing any lab work. Using chloramine to treat microbial growth with an expectation of sterilization shows a lack of basic knowledge. apparently, no one in the lab is helping you - so stop doing the work until you've established basic knowledge of micro and how to work with microbes.
jorge1907,
in the forum rules it says "1. No personal attacks."
Telling people who are already working in the lab to stop working is in my eyes a personal attack.
Not everybody is lucky to get a proper education in the lab from the beginning; some have to learn lots of basic things on their own!
I have seen lots of people coming to the lab with nearly no knowledge about microbiology, although they were making their master or even PhD. But the ones who were "brave" enough to ask basic questions were leaving the lab and you don't have to be afraid if they work somewhere else. These people are no danger to themselves and their lab mates. But some of the ones who were not asking questions (for which reason ever) I would not like to work with....as they were making still lots of dangerous mistakes (such as you can throw everything down the drain even highly toxic substances)!
So please don't discourage people from asking "stupid" basic questions! These basic questions make the lab a safer place for all of us! (Getting a bit pathetic now, but I am sure you got my point
)gebirgsziege
This is not a personal attack - it is a strong recommendation that folks untrained in science get appropriate training before risking themselves and others near them. Folks benefit much more from basic knowledge that lets them learn safely more than the often ill-informed and misdirected comments usually offered. You suggest autoclave parameters and practices. Nice - IF the lab were under supervision of someone who knew micro, the poster wouldn't be using chloramine for this purpose in the 1st place. With that limited level of expertise, veronika is likely to injure herself or others trying to comply.
Again she needs basic training.
Is that clear?
Thanks for both suggestions, both are true. I am working with yeast cells for a longer time - doing the light microcopy, electron microcopy and now even high pressure freezing of the cells, and I try to learn everything that is possible to learn about the yeast and the techniques for their preparation (but it is a really hard work as you can conclude from my "basic question"). But it is somethimes really hard, when nobody else could tell you how the yeast cells behave, what is recommended to to and what is avoided to do... and so on. I do not know if you can understand me well, but... I like my work very much, sometimes I am really deseapered when I see, that something is totally different from the cell cultures preparation and I dont know whats going wrong... I tried to find many times something about the "killing of yeast cells" using the different "topics" for searching...but I did not find anything. And finally, I log in to this bioforum I find the answers for my questions:))) 
And these where the only two questions from thousands:)))))
So, after that horrible discovery I start to autoclave the yeast cells ... and they still have some post-mortem movement in the medium... am I crazy or are they immortal?
Neverthelles, only to vindicate myself....I was really sure, that cells are dead after the treatment with chloramine.... (( I know what does it mean... I am stupid, it is my mistake that I do not have "adequate" knowledges ... I am going to look for other basic knowledges.... 
They neither move nor are they immortal.
Please veronkia, stop playing at this and gt some professional direction and instruction in your work.
I have been reading the forum for a period here now and I do wonder how it is possible that so many people ask very basic questions about things they should really know.
I cant understand that they are just allowed to work in the lab without any supervision when they are all new to it.
When I started working in the lab someone followed me the whole day and explained me everything I needed to know and if I didnt knew a thing after that day, I just asked the people working there.
And only when they didnt know it, I would ask it here or somewhere else.
I do not understand why a lot of people do not seem to be able to ask basic stuff at their supervisors or co-workers.
Nor do I understand why they are allowed to work in a lab without any basic training, or without any senior that has worked with the products before.
Veronika I sure do hope you are not working with Candida or anything like that...
Quite simple actually, because nobody in the lab or department is working on the same organsim. I have seen several examples where the PI doesn't understand the organsim they have asigned to their PhD student. Said student is suppose to learn everything they need to get the idea done.
I have even withness a case where a PhD student from a protein lab was asked/order to make a protein mutant and left to her own devises how to go about doing so. No help was given, because the PI was busy and her PI felt, that she, as an adult, should old enough be able to go out and learn what every she needed to know. He wasn't going to hold her hand. Harsh but somewhat logical.
So there are few PI who aren't all interested in teaching, and there are some who are not knowledgable enough about the technical aspects of the host organism or subject to teach you. (Even if you are being directed to use said organism).
I don't know about other unis, but in my uni basic traning is only given for radioisotope handling and ultra centrifuge loading. Everything else is dependent of inhouse knowledge of your lab and finding friends who will take the time to share their skills.
I understand what you are saying, get more basic traning. But I also understand Veronika24 situation. For 2/3 of my PhD, I was the only person in the department working on yeast of any sort. The only person who could advise me on the technical side were books in the library and the Internet.
I have even withness a case where a PhD student from a protein lab was asked/order to make a protein mutant and left to her own devises how to go about doing so. No help was given, because the PI was busy and her PI felt, that she, as an adult, should old enough be able to go out and learn what every she needed to know. He wasn't going to hold her hand. Harsh but somewhat logical.
So there are few PI who aren't all interested in teaching, and there are some who are not knowledgable enough about the technical aspects of the host organism or subject to teach you. (Even if you are being directed to use said organism).
I don't know about other unis, but in my uni basic traning is only given for radioisotope handling and ultra centrifuge loading. Everything else is dependent of inhouse knowledge of your lab and finding friends who will take the time to share their skills.
I understand what you are saying, get more basic traning. But I also understand Veronika24 situation. For 2/3 of my PhD, I was the only person in the department working on yeast of any sort. The only person who could advise me on the technical side were books in the library and the Internet.
I find this very very strange.
I can understand that most of the people that start working a a lab, and certainly those doing a phd are smart and capable of learning things from books etc...
But still this does not mean that they can simply start working with the equipment and microorganisms without any help, guidance.
(especially when they did a more theoretic study, master, where they did not see a lot of lab work)
From my own experience I have noticed that when someone explains it to me and shows it, I'll learn it a lot faster and understand it.
Even if there is a manual with the machine, I find it more relaxing and better to learn it from someone who used it.
Besides that, a lot is just not that clearcut in books. I mean, you can find lots and lots of info , but the right, exact info for the job? It could take ages to figure it out and you can still be doing something bad.
Sometimes its all in the details...
I find this a very strange thing.
Maybe I was always lucky to met people that did help me and showed me everything? I dunno, but I sure do it stays like this.
I think that when they have no one that ever worked with the thing the student has to work , they should send the student to another lab to learn or send him/her to a studyday or whatever.
At the end it would save the student a lot of time and even a lot of money because he or she will not make any (or less) mistakes.
Dear all,
hard to say something for justification. I know that its necessery to obtain basic theoretical and also practical information about the problem that you have to face up. It was also my aim...and still is. I came to the lab, my boss said me what will be my project for my PhD thesis...and that was all. Most of the time he is not in the lab, once the months he wants to see my results and thats all. As I said nobody in our lab has an experiences with the yeast cells and the only person that showed me how to do some "basic" methods (with culture cells) was our laboratory technician... Everything else I had to learn by my own from the internet. Before the entering the lab, i had no experiences with lab work and preparation of specimens for LM or EM. So for me it was a really new world (in the school we had only few practicals).
Now I think that I learn a lot by my own, but as I see not everything. And it takes a lot of time. Here, new people entering our lab have no luck to have somebody next to them who will tell them what to do and how to do.... You have to do everything alone, because everybody has a lot of "own" work. Nobody is interested in you (( No collaboration:(( And I think that it is not in my hand to resolve this problem....
So, please if you belong to the "old" generation of scientist...try to give as lot of informations as you can to the younger colleagues And be care about the relationships in the lab
And that all about the problem how to kill the yeast cells
I understand your dilemma. Go to the micro dpt (or a microbiologist in your bio dpt) and get the training.