calculation of qRT-PCR - to Roche LC480 users (May/27/2008 )

Hello

can you please explain me ........when using Roche LC 480 how it calculates the CP value and the mean conc. I mean what is the formula behind the calculated result.

thanks in advance

-T. reesei-

QUOTE (T. reesei @ May 27 2008, 03:08 PM)

Hello

can you please explain me ........when using Roche LC 480 how it calculates the CP value and the mean conc. I mean what is the formula behind the calculated result.

thanks in advance

If you aren't using the fit point method (with a standard curve) google second derivative maximum.

Cheers,
maset

-maset-