LDH Assay - how to analyze & present data (May/26/2008 )

Hi all,

Wondering what's the most widely used method for presenting LDH cytotoxicity data? I've recently done this assay but am unsure of how I should show my results.

A friend told me to calculate using this formula which I'm not quite convinced; would like some opinion:

eg. normoxic control = 0.3
test compound A = 0.4
test compound B = 0.5 ( which is more toxic than A)

For A = % Normoxic control = (0.4-0.3)/0.3 = 33% of normoxic ctrl
B = % NC = (0.5-0.3)/0.3 =66% of normoxic ctrl (which didnt really make sense to me; since B is more toxic than A )

a little puzzled and confused

Would appreciate if you guys can share how you do it?

Thanks in advance!



shyn

While somebody is busy writing a specific answer, you may want to dig into these results:

http://search.vadlo.com/b/q?p=1&sn=158...rel=0&srt=0

..

Hi,

Thanks to CellCounter and TheBearer for your help! I've decided to takeup TheBearer's suggestion and express the results as % cytotoxicity.
CellCounter-- The link you provided was wonderful! there's alot of info on LDH assay, thanks!