Methylase negative strains? - (May/22/2008 )
Hello....
-Neko Tonegawa-
QUOTE (Neko Tonegawa @ May 22 2008, 01:16 PM)
Hello....
I would like to know if TOP10F' or JM109 are methylase negative strains...
If I transform this cells with methylated plasmid, can I get un-methylated plasmid?
thanks....
(new to this forum >_<)
-Neko Tonegawa-
welcome!
JM109 and TOP10F' are not methylation deficient strains, DNA in these cells will be methylated (check http://openwetware.org/wiki/E._coli_genotypes for E coli genotypes)
we normally use the INV110 strain from invitrogen to obtain non-methylated DNA
-dpo-
QUOTE (dpo @ May 22 2008, 02:01 PM)
welcome!
JM109 and TOP10F' are not methylation deficient strains, DNA in these cells will be methylated (check http://openwetware.org/wiki/E._coli_genotypes for E coli genotypes)
we normally use the INV110 strain from invitrogen to obtain non-methylated DNA
JM109 and TOP10F' are not methylation deficient strains, DNA in these cells will be methylated (check http://openwetware.org/wiki/E._coli_genotypes for E coli genotypes)
we normally use the INV110 strain from invitrogen to obtain non-methylated DNA
Thank you! that was very useful n_n
-Neko Tonegawa-