i was confused about ChIP result - i need help (May/17/2008 )
sorry for no pic
        i use two pairs of primer to run pcr after DNA extraction, the positive primer 's product was good ,that is Ab got band ,wAB no band ,and input got band . but the result for the other primer was not satisfying, cause ab and input got bands,but wab got band too(thinner),  why ? 
        i used the same template
        i'm studying the transcription factor-promoter reaction?  is it possible that the same TF binds differently for different promoter? 
        what is the most possible reason? 
        any help would be appreciated 
-yww-pride-
First, there is some nonspecific pulldown of DNA in your sample that can only be amplified by your 2nd pair of primers becuase it is more efficient than the first pair.
-pcrman-
