Getting RNA and Protein out of/off from Matrigel - (May/14/2008 )

Hi everyone,

I am trying to figure out a way to get cells out of Matrigel after they have undergone channel formation to try and run PCR and Western blots. Does anyone know how to get the cells off without taking a bunch of Matigel off with them, or some way to make a cell pellet from this? I just keep getting Matrigel in with the cells when I look at them under the microscope. Thanks very much.

Lauren

I don't do matrigel, but you may want to find a way on how to dissolve it without killing your cells.

See if these protocols for protein isolation work for you. RNA search didn't bring up any obvious ones!

http://search.vadlo.com/b/q?sn=158621799&a...otein&rel=0

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Oops.. I did it again and it worked! May be this thing is expanding :-) Here is RNA:
http://search.vadlo.com/b/q?sn=158621799&a...l+RNA&rel=0
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