Magnetic stirr bars - cell culture (May/08/2008 )
Hello all,
Are there magnetic stirr bars apropriate for cell culture (single-use, steril and LPS-free)?
thxs
-BioPe-
Why would you want to use one? They would probably damage the cells while stirring. I would use a shaking table/incubator to agitate media instead.
-bob1-
don't stir.
shake the media. Much better at air-ating the media.
V
-vetticus3-
QUOTE (BioPe @ May 8 2008, 06:55 AM)
Hello all,
Are there magnetic stirr bars apropriate for cell culture (single-use, steril and LPS-free)?
thxs
Are there magnetic stirr bars apropriate for cell culture (single-use, steril and LPS-free)?
thxs

Dear BioPe,
The best way to grow cells in suspension is using Stirrer bottles.....MADE SPECIFICALLY FOR MAMMALIAN CELL SUSPENSION CULTURE. Advantages:
Reusable borosilicate, Autoclavable bottles....125, 250, 500 and 2000ml available
Fine control of Revolution speed..i.e. reduces shear stress.
Easy to passage......no trypsin required.
Reliable
I use Techne stirrer bottles available from Barloweworld.
Regards
Rhombus
-Rhombus-