The Best way of merging phase-contrast and fluorescence images of cells - in Photoshop (May/03/2008 )

Hello,

What is the best setting for merging a phase-contrast images and a GFP image of cells in photoshop. I know I need to have the two images on two layers, but what is the best blending mode and which layer should at top?

thanks.

Hello

I would not use photoshop for this, there are plenty of caveats, but basically, photoshop is not a program designed for science imaging (it can alter multiple parameters in non-lineal ways). You are best off using ImageJ and playing around with image calculator (add image) and altering brightness and contrast to the whole image. Referees can´t fault this but you should stick to clear guidelines (see J. Cell Biol What´s in a picture? 166(1) 11-15).