protein extraction from Daphnia/rotifera - (May/01/2008 )
Hi,
I would like to extract proteins, but i do not know what lysis buffer i need to use. i tried to use triton x-100, but someone told me that this is not enough. do you have maybe a recipe for lysis buffer for this kind of animals?
-tamar-
QUOTE (tamar @ May 1 2008, 09:07 AM)
Hi,
I would like to extract proteins, but i do not know what lysis buffer i need to use. i tried to use triton x-100, but someone told me that this is not enough. do you have maybe a recipe for lysis buffer for this kind of animals?
I would like to extract proteins, but i do not know what lysis buffer i need to use. i tried to use triton x-100, but someone told me that this is not enough. do you have maybe a recipe for lysis buffer for this kind of animals?
I do not work with Daphnia (magna) but I know that it is a model for hemoglobine research; try to find recent papers in this field as protein extraction is therein a main method
-The Bearer-
QUOTE (The Bearer @ May 13 2008, 03:07 PM)
QUOTE (tamar @ May 1 2008, 09:07 AM)
Hi,
I would like to extract proteins, but i do not know what lysis buffer i need to use. i tried to use triton x-100, but someone told me that this is not enough. do you have maybe a recipe for lysis buffer for this kind of animals?
I would like to extract proteins, but i do not know what lysis buffer i need to use. i tried to use triton x-100, but someone told me that this is not enough. do you have maybe a recipe for lysis buffer for this kind of animals?
I do not work with Daphnia (magna) but I know that it is a model for hemoglobine research; try to find recent papers in this field as protein extraction is therein a main method
-tamar-
QUOTE (tamar @ May 14 2008, 09:36 AM)
QUOTE (The Bearer @ May 13 2008, 03:07 PM)
QUOTE (tamar @ May 1 2008, 09:07 AM)
Hi,
I would like to extract proteins, but i do not know what lysis buffer i need to use. i tried to use triton x-100, but someone told me that this is not enough. do you have maybe a recipe for lysis buffer for this kind of animals?
I would like to extract proteins, but i do not know what lysis buffer i need to use. i tried to use triton x-100, but someone told me that this is not enough. do you have maybe a recipe for lysis buffer for this kind of animals?
I do not work with Daphnia (magna) but I know that it is a model for hemoglobine research; try to find recent papers in this field as protein extraction is therein a main method
Thanks! i found something- hope it will be good for rotifers too.
-tamar-