How to do this technique using culture procedures? - (Apr/17/2008 )
I want to study the effect of a certain drug on the blood and some organs of the mouse.
how can i benefit from the culture method in order to stydy it in vitro????
i need information about how to do culture for blood or any other tissues????
-Jehane-
QUOTE (Jehane @ Apr 17 2008, 12:28 PM)
I want to study the effect of a certain drug on the blood and some organs of the mouse.
how can i benefit from the culture method in order to stydy it in vitro????
i need information about how to do culture for blood or any other tissues????
how can i benefit from the culture method in order to stydy it in vitro????
i need information about how to do culture for blood or any other tissues????
most organs, or let´s say tissues have model cells to study in vitro; you may start with primary cell culture; oncogenic cell lines are often used cells as they are, in most cases, easy to handle and immortal
-The Bearer-
QUOTE (The Bearer @ Apr 17 2008, 01:18 PM)
most organs, or let´s say tissues have model cells to study in vitro; you may start with primary cell culture; oncogenic cell lines are often used cells as they are, in most cases, easy to handle and immortal
i want mor detail information if you could help me in this topic as i'm new in this field.
-Jehane-
QUOTE (Jehane @ Apr 17 2008, 01:47 PM)
QUOTE (The Bearer @ Apr 17 2008, 01:18 PM)
most organs, or let´s say tissues have model cells to study in vitro; you may start with primary cell culture; oncogenic cell lines are often used cells as they are, in most cases, easy to handle and immortal
i want mor detail information if you could help me in this topic as i'm new in this field.
you may offer the organs/tissues/cells you really want to analyze, so that we can open discussion in more detail; I regret that I cannot recommend for blood cells; the only one I use are Jurkat cells (T-cells)
-The Bearer-
QUOTE (The Bearer @ Apr 18 2008, 06:56 AM)
you may offer the organs/tissues/cells you really want to analyze, so that we can open discussion in more detail; I regret that I cannot recommend for blood cells; the only one I use are Jurkat cells (T-cells)
thank you so much for ur help
my problem is that i study the effect of some drugs on the mutation of some genes within rat genome. but i study this effects in vivo. and get the DNA from blood lymphocytes.
now i want to study those effects in vitro.
so what could i do now??
doing culture for lymphocytes??
then what????
best regards.
-Jehane-