Importance of volume of Flascon tubes - (Aug/22/2004 )

Hi !!
I am wondering if the volume of the flacon tubes used to make your colonies grow after picking them from plates affect their grow or not.
I use to make my bacteria grow in 10ml LB, do you think it makes a difference to do it in 14ml or 50ml Flacon tubes ?
Thanks for your answers.
Anne

Hi,
the area that you are growing your bacteria in makes a difference to the yield, I would definitely go with the 50ml tubes. Obviously this is going to increase your amount only, not give you growth if you have none:)

the usual concept is, that the flask you grow your bacteria in should have at least 5 time the actual volume of you culture.

mike