Taq polymerase - (Apr/09/2008 )

Does taq polymerase have proof reading activity.
I think pfu polymease have proof reading activity

---

Normal taq does not have proofreading abilities and is commonly used for random mutagenesis or pcr screens. Companies now sell mixtures of polymerases where they mix taq with others that contain proofreading polymerases that fix the errors taq creates. PFU does have fairly good proofreading, although I just got sequences back where I used PFU Turbo and half had single point mutations.

---