Non-growth of E. coli TOP10F' in LA+tetracicliyn media - (Feb/28/2008 )

I transformed some E. coli TOP10F' cells with pUC19.
The transformed cells were plated in LA+tetracyclin+ampicilyn and i had some growth but i coudn't see any satalite colonies.
My control cells that were plated in both LA+tetracyclin and LA+tetraciclyn+ampicilyn didn't grow. I didn't have a single colony!
In both cases the cells were incubated at 30ºC for 30-40 min in LB.

Can somebody help me find an explanation for the lack of growth??

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Your Top10 F' cells should grow on the tet only plates. If they do not, they either never had, or have lost, their F plasmid. Are you sure they are F' rather than plain Top10?

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They were provided by a professor and he garanteed the presence of the F plasmid. Besides the transformed cells came from the same culture and they were able to grow with tet present.

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