Polybrene concentration-toxicity - (Feb/23/2008 )

Dear All,

I used a wrong concentration of Polybrene for my retroviral infection of MEFs. I was supposed to use 8ug/ml final concentration of Polybrene in the virus supernatant, but the actual concentration is 40ug/ml, which is 5 times higher. I know polybrene may be toxic to cells at higher concentration, but is 40ug/ml safe? I haven't observed obvious cell death 8 hours after the infection.

Thanks a lot!

Dadi

This must be your lucky day. Good if there is no toxicity in 2 hrs.

We see toxicity with 8ug/ml in 24 hrs. All of our cells die.