TBE - (Feb/16/2008 )
Hello,
I'm wondering, what is the purpose of EDTA in TBE buffer? I ask because I accidentally added twice as much EDTA. I had to scale down my reaction by a factor of two because we didn't have enough tris. Now I have imperfect buffer and no Tris!!
PS. The pH of my stock (5x) is 8.3, which is what is expected for 5xTBE.
-stemcell69-
try making SB buffer... runs just like TBE but faster and uses no tris.
for 20X
8g NaOH
45g Boric acid
1l water
should be about pH 8 when made up.
-bob1-
Excellent! Thank you Bob!
-stemcell69-