media composition - (Feb/11/2008 )

hello friends
i need help in making this media
Myelocult human stem cell long term culture:
•Horse Serum
•Fetal Bovine Serum
•i-Inositol
•Folic Acid
•2-Mercaptoethanol
i get the components from the internet but i couldnt find the exact concentrations of each one of them ? any help?
thankx

up
needed urgently
thankx

Myelocult HCC-5100 appears to be a trade name for a culture medium from Stem Cells Technology. You probably will not find the exact composition listed. The primary literature on isolation and culture of the cells you are using will probably give you an idea of the composition you need. But you might be better served by purchasing the medium rather than trying to make it from scratch.

mmm...Thankx phage
i know its better to buy it but i checked and iys really expensive so thats why my professor said to try and make it.
still hoping

Do you know what company the cells came from? If you call them they should give you the necessary components and concentrations.

- Eli

MYELOCULT H5100
MYELOID LONG-TERM CULTURE MEDIUM
FOR PRIMITIVE HUMAN HEMATOPOIETIC CELLS

PRODUCT DESCRIPTION:
This medium contains horse serum (12.5%), fetal bovine serum (12.5%) and 2-mercaptoethanol
(10-4 M) in alpha MEM supplemented with L-glutamine (2 mM), I-inositol (0.16 mM) and folic acid
(16 μM). These components have been pretested and selected for their ability to optimally initiate and
maintain myelopoiesis in long-term cultures of human bone marrow.1-5
Half-media changes at weekly intervals should sustain myelopoiesis for at least 5 - 8 weeks.

Also, check out the PDF:

http://www.stemcell.com/technical/05100_05150-PIS.pdf

THANK U SOOOOOOOOOOOOOOO MUCH