G protein inhibition - (Feb/08/2008 )

Hi!

Is there another way I can eliminate G-protein-dependent signaling other than inhibitors, siRNA and Gq-protein knockout transfected into cells?

We've tried transfecting the G-protein knockouts into MEF cells but only get ~5% transfection... has anyone got any ideas, suggestions?

Thanks!

Zoe

-zoe8-

QUOTE (zoe8 @ Feb 8 2008, 05:47 AM)

Zoe

transfection of dominant-negative forms

-The Bearer-

QUOTE (cupidstunt @ Feb 29 2008, 06:56 AM)

hi,

try using pertusis toxin which inactivates G-proteins except Gq.

Pertussis toxin is limited only to inhibition of Gi, but not G12, G13, Gq and all the other G proteins.

-aleruiz-

QUOTE (zoe8 @ Feb 8 2008, 12:47 PM)

Zoe

hi,
try using cell lines not having g-proteins like insect cell ines.

-cupidstunt-