how to remove contaminated RNA in DNA extract - (Feb/04/2008 )
I used phenol:chlorform method to extract my genomic DNA, but forgot to put RNAase along with proteinase.
After extraction, 260/230 ratio is around 0.66, 0.60 and 260/280 ratio is around 1.81 and 1.71.
Does that mean much contaminated RNA should be in the DNA extract?
Is there any method for RNA removal after DNA extraction?
-cameejy-
looks more like phenol and/or carbohydrate contamination. your 260/280 looks good.
if it's phenol contamination you can do a chloroform extraction.
-mdfenko-
QUOTE (mdfenko @ Feb 4 2008, 03:10 PM)
looks more like phenol and/or carbohydrate contamination. your 260/280 looks good.
if it's phenol contamination you can do a chloroform extraction.
if it's phenol contamination you can do a chloroform extraction.
Thanks. is there a method i can remove RNA in already extracted DNA?
-cameejy-
treat with dnase-free rnase then re-extract with phenol/chloroform.
-mdfenko-
QUOTE (mdfenko @ Feb 5 2008, 01:28 PM)
treat with dnase-free rnase then re-extract with phenol/chloroform.
dnase-free rnase treatment is the best i think .........
if much worried then you can go for PEG treatment
-T. reesei-