RNA extraction from Bone Marrow - (Jan/21/2008 )

hi there
i am going to perform an RNA extraction from bone marrow though am finding it difficult to find evidence from people who have done this. i am using Qiagen method, though also have trizol available... can anyone advise? the bone marrow has been extracted and frozen and is being stored at -80 celcius
thanks alot
emily

QUOTE (emily kewley @ Jan 21 2008, 03:56 PM)

hi there
i am going to perform an RNA extraction from bone marrow though am finding it difficult to find evidence from people who have done this. i am using Qiagen method, though also have trizol available... can anyone advise? the bone marrow has been extracted and frozen and is being stored at -80 celcius
thanks alot
emily

I have used Trizol to extract RNA from BM. Just thaw your sample of ice and then resuspend in the required amount of Trizol and off you go

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thanks so much. i guess it would work with the Qiagen method too - have you heard of this? otherwise, i may just stick to trizol as have used it before too.
emily
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Do you mean the Qiagen kit (comes in a red box)? I have never used it, but from memory you would use it if you had a small amount of cells?

hey
yes, the red box. not sure about the small amount of cells??
thanks though