Isolation entire gene from degenerate primer - isolation of entire gene (Jan/19/2008 )
Asalam alicom (Peace for you )
Dear guys
I have primers of genes in cluster designed from conserved DNA sequence in center and end of gene (s)
I want to isolate whole gene from these primer which anneal in center and end and dont lose any sequence of gene
I want techique to isolate entire gene
Thank you
I waiting your opinion and experience
Regards
Ahmed
-hamadh83-
you can use the primers you have to sequence the entire gene (designing intermediate primers as necessary). then you can design the other end primer and pcr the entire gene.
-mdfenko-