Is this gene going to express? - is only the firs ATG read and express or all 3 reading frame? (Jan/10/2008 )
QUOTE (aztecan princess @ Jan 11 2008, 04:42 PM)
Other Question…. We are cloning viral genes for expression like this gene. This genes don’t have a native kozak sequence. Do you think we would add it to have a better expression, or they would be expressed without problem?
If the virus use the cell‘s machinery (eucariotic) to express their genes in nature, I think the kozak sequence won’t be necessary for express the same genes in an expression vector. But I think may be adding kozak sequence would increase the expression level.
What do you think????????
If the virus use the cell‘s machinery (eucariotic) to express their genes in nature, I think the kozak sequence won’t be necessary for express the same genes in an expression vector. But I think may be adding kozak sequence would increase the expression level.
What do you think????????
In our viral vectors, we always add the kozak sequence for better expression.
-scolix-