Radioactive hybridisation - Labelling the nucleotides (Jul/05/2004 )
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Why is that most of the labs use alpha P32 labelled dCTP for labelling?
Is ther any specific reason for using this label?
Plz reply!
-mishla-
Mishla,
People usually use dCTP to label all along a DNA strand as it is normally alpha labelled and dATP just to label at the 5' Phosphate group as it usually is gamma labelled.
I hope it helps
Yasmine.
-Yasmine-