Affymetrix chip - unexpressed genes, which signal cuttof to use - (Dec/17/2007 )
Dear forum users,
I am using hgu133plus2 chips. I would like which intensity treshold should be used for filtering out genes that are not expressed at all.
tx
karpatov
-karpatov-
To my knowledge,
Affymetrix produces its own present or absent call, by comparing the density coming from mismatch probes with that of perfect match probes.
So, rather than setting up a threshold, I would recommend having the present/absent calls. And then, eliminating the genes for which all the samples called absent.
Regards
-ersenkavak-